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Article Publish Status: FREE
Abstract Title:

HPLC-PDA-MS/MS profiling of secondary metabolites fromcladode, peel and fruit pulp extracts and their antioxidant, neuroprotective effect in rats with aluminum chloride induced neurotoxicity.

Abstract Source:

Saudi J Biol Sci. 2020 Oct ;27(10):2829-2838. Epub 2020 Jul 9. PMID: 32994743

Abstract Author(s):

Seham S El-Hawary, Mansour Sobeh, Wafaa K Badr, Mohamed A O Abdelfattah, Zeinab Y Ali, Mona E El-Tantawy, Mohamed A Rabeh, Michael Wink

Article Affiliation:

Seham S El-Hawary

Abstract:

(L.) Mill. (OFI), also known as Indian figor prickly pear, is a member of the family Cactaceae that produces edible, nutritionally rich sweet fruits. It has been traditionally used to treat several health disorders and is considered to possess various therapeutic properties. In this work, we have characterized 37 secondary metabolites using HPLC-MS/MS, identified the polysaccharide from the fruits and cladodes pulpand estimated the neuroprotective activityAll tested extracts exhibited substantial antioxidant activitiesand neuroprotective potential in AlClinduced Alzheimer's condition. Administration of OFI extracts attenuated AlClinduced learning and memory impairment as confirmed from passive avoidance test and counteracted the oxidative stress as manifested from decreasein the elevated MDA level, increased TAC, GSH, SOD and CAT levels. OFI extracts significantly decreased the elevated brain levels of proinflammatory cytokines (NF-κβ and TNF-α), increased anti-inflammatory cytokine (IL-10), and monoamine neurotransmitters (NE, DA, 5-HT) as compared to positive control group. The extracts showed a significant decrease in acetylcholinesterase level (AChE) as compared with AlClFurthermore, molecular docking was performed to investigate the ability of the major constituents of OFI extracts to interact with acetylcholinesterase (AChE) and serotonin transporter (SERT). Among the tested extracts, cladodes contain highest phenolic content and exhibited the highest antioxidant, anti-inflammatory and neuroprotective activities, which could be attributed to presence of several polyphenols, which could function as AChE and SERT inhibitors.might be promising candidate for treating Alzheimer disease, which makes it a subject for more detailed studies.

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