Abstract Title:

Application of PCR for detection of mycoplasma DNA and pestivirus RNA in human live viral vaccines.

Abstract Source:

Biologicals. 1996 Dec ;24(4):371-5. PMID: 9088554

Abstract Author(s):

T Sasaki, R Harasawa, M Shintani, H Fujiwara, Y Sasaki, A Horino, T Kenri, K Asada, I Kato, F Chino

Article Affiliation:

T Sasaki


PCR techniques were applied for the detection of mycoplasma DNA and pestivirus RNA to 43 lots of live viral vaccines (measles, mumps, rubella, and oral poliomyelitis) produced by six manufacturers in Japan. Although mycoplasma DNA was not detected in any of the vaccines tested, pestivirus RNA was detected in 12 lots (28%). The incidence of contamination among the four viral vaccines was in the range of 20 to 37%, and the incidence among the six manufacturers varied from 0 to 56%.

Study Type : In Vitro Study

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