Article Publish Status: FREE
Abstract Title:

Healing Process of Rat Skin Wounds Treated With Vitamin C and Low-Intensity Laser Therapy.

Abstract Source:

Cureus. 2020 Dec 6 ;12(12):e11933. Epub 2020 Dec 6. PMID: 33304712

Abstract Author(s):

Luis Flavio Duraes Gomes Oliva, Doroty Mesquita Dourado

Article Affiliation:

Luis Flavio Duraes Gomes Oliva


INTRODUCTION: This study evaluated, from a histological point of view, the process of repairing skin wounds caused in the dorsal region of rats when subjected to treatment with vitamin C, low-intensity laser, and association of both.

METHODS: Forty-eight adult male rats (, albinus, Wistar), weighing between 250 and 300 g were used in this study. The rats were anesthetized with sodium pentobarbital (10 mg/kg) intraperitoneally (IP) and a circular area of​​skin of approximately 8 mm in diameter was removed from the dorsal region of their back by a punch. The animals were randomly divided into four groups of 12 individuals: Group I, control group, was treated with saline solution; Group II was treated with topical application of vitamin C; GroupIII was treated with low-intensity laser; and Group IV was treated with both low-intensity laser and topical application of vitamin C. Samples were histologically analyzed through optical microscopy with hematoxylin and eosin staining and collagen I and III concentrations were quantified using the picrosirius-hematoxylin histochemical method and further submitted to statistical analysis.

RESULTS: Whilst the control and vitamin C groups admittedly showed slight epithelial proliferation at the wound edges, the group irradiated with low-intensity laser and the group treated with both laser and vitamin C had already partially formed epidermis, with a more organized underlying connective tissue and less evident inflammatory process. The group treated with laser alone obtained a higher concentration of type I collagen fibers and the group with the highest amount of type III collagen fibers was the one treated with the association of vitamin C and laser.

CONCLUSION: The present findings suggest that in spite of all treatments being effective in the repair of skin wounds compared to the control group, the isolated use of low-intensity therapy laser and its combined use with topical vitamin C showed the most favorable results, indicating that those could be further used for the treatment of skin wounds.

Study Type : Animal Study

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