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Abstract Title:

Catechin isolated from faba beans (L.): insights from oxidative stress and hypoglycemic effect in yeast cells through confocal microscopy, flow cytometry, andstrategy.

Abstract Source:

J Biomol Struct Dyn. 2021 Jun 30:1-11. Epub 2021 Jun 30. PMID: 34192480

Abstract Author(s):

Dhiraj Kumar Choudhary, Navaneet Chaturvedi, Amit Singh, Abha Mishra

Article Affiliation:

Dhiraj Kumar Choudhary

Abstract:

The present aim of this investigation was to evaluate the effect of catechin from faba beans on oxidative stress and glucose uptake in yeast cells. Flow cytometry approach indicated that 2-NBDG (1.98 ± 0.37) seed extract had a lower relative fluorescence signal than methanol (5.98 ± 0.67) and acetone seed extract (4.43 ± 0.55). In comparison to the control and seed extract, HOexposure increased the apoptosis rate of yeast cells from 8.20% to 64.80%. Yeast cells incubated with HOproduced significantly more ROS intensity (162 ± 4.32, < 0.05) than control cells (118 ± 2.52, < 0.05) and less than seed extract-treated cells. Molecular dynamics simulation studies were performed for cat:α-amylase (catechin-α-amylase complex) which revealed the stable and mixed mode of inhibition during a simulation. The synergistic action of polyphenols or catechin present in seed extract may be responsible for the anti-oxidative stress and hypoglycaemic effects. The findings of this study may provide insight into the further development of a novel antidiabetic drug for T2DM. Communicated by Ramaswamy H. Sarma.

Study Type : In Vitro Study

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