Abstract Title:

Antibiofilm and Anti-Quorum sensing Activities of Eugenol and Linalool From Ocimum tenuiflorum against Pseudomonas aeruginosa Biofilm.

Abstract Source:

J Appl Microbiol. 2021 Jun 2. Epub 2021 Jun 2. PMID: 34077580

Abstract Author(s):

Dibyajit Lahiri, Moupriya Nag, Bandita Dutta, Soumik Dey, Dipro Mukherjee, Sanket J Joshi, Rina Rani Ray

Article Affiliation:

Dibyajit Lahiri


AIMS: Determination of the ability of two bioactive compounds, namely Eugenol and Linalool, purified from leaves of Ocimum tenuiflorum for eradication of biofilm produced by Pseudomonas aeruginosa.

METHODS AND RESULTS: The phyto-extract of Ocimum tenuiflorum (KT), a common ethno-botanical plant of India, was purified through HPLC, and was analyzed using UV spectroscopy and GC-MS. Eugenol and Linalool were found to be the most active amongst all phytocompounds present in phytoextract and showed a significant reduction in the viability of sessile cells of Pseudomonas aeruginosa and the minimum revival after withdrawal of phyto-challenge. They could bring about notable reduction in the protein and carbohydrate content of exopolysaccharide of biofilm . Eugenol and linalool could affect the synthesis of QS proteins like lasA and lasB as well as virulence factors such as pyocyanin, and rhamnolipids, which seriously hamper the formation of biofilm. The biofilm framework was extremely affected by the phytocompounds through the reduction of protein and carbohydrate content of EPS. Another interesting find out was that they brought about maximum inhibition to the genomic DNA and RNA content. The studies were supported by in-silico interaction between eugenol and linalool with the QS proteins. The antibiofilm efficacies of eugenol, linalool and phytoextract (KT) were further confirmed by microscopic studies with scanning electron (SEM), Atomic Force (AFM) and Fluorescence confocal (FCM) microscopic studies.

CONCLUSIONS: The phytocompounds are proved to be more effective than conventional antibiotics in inhibiting the biofilm forming sessile cells and can be used as a replacement for antibiotic.

SIGNIFICANCE AND IMPACT OF STUDY: Pure eugenol extracted from common basil leaves can be used as a safe substitute for common antibiotic for treatment of chronic infections caused by Pseudomonas aeruginosa. It will be cost effective, devoid of notable side effects and will not generate antibiotic resistance in host body.

Study Type : In Vitro Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2021 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.