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Abstract Title:

Autophagy efficacy and vitamin D status: Population effects.

Abstract Source:

Cell Immunol. 2020 Feb 28:104082. Epub 2020 Feb 28. PMID: 32241530

Abstract Author(s):

Abhimanyu, Vanessa Meyer, Brandon R Jones, Liza Bornman

Article Affiliation:

Abhimanyu

Abstract:

Toll-like receptor (TLR) 2/1 signalling is linked to autophagy through transcriptional actions of the 1,25-dihydroxyvitamin D(1,25(OH)D)-vitamin D receptor (VDR) complex. Population-specific effects have been reported for TLR2/1-VDR signalling. We hypothesized that population effects extend to autophagy and are influenced by vitamin D status. Serum 25(OH)Dof healthy South Africans (Black individuals n = 10, White individuals n = 10) was quantified by LC-MS/MS. Primary monocytes-macrophages were supplemented in vitro with 1,25(OH)Dand stimulated with the lipoprotein PamCysSerLys. TLR2, VDR, hCAP18, Beclin1, LC3-IIB, cytokines and CYP24A1 mRNA were quantified by flow cytometry and RT-qPCR, respectively. Black individuals showed significantly lower overall cumulative LC3-IIB (P < 0.010), but higher Beclin1, VDR, IL6 and TNFA (P < 0.050) than White individuals. 1,25(OH)Denhanced autophagic flux in monocytes-macrophages from Black individuals upon TLR2/1 stimulation and strengthened autophagy in 25(OH)Ddeficient individuals (independent cohort, n = 20). These findings support population-directed vitamin D supplementation.

Study Type : Human Study
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Sayer Ji
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