Abstract Title:

Fuel smoke condensate induced DNA damage in human lymphocytes and protection by turmeric (Curcuma longa).

Abstract Source:

Mol Cell Biochem. 1990 Jun 1;95(1):21-30. PMID: 2366750

Abstract Author(s):

V K Shalini, L Srinivas

Article Affiliation:

Department of Nutrition and Food Safety, Central Food Technological Research Institute, Mysore, India.


Twigs-dry leaves smoke condensate (TDS) was investigated for its DNA damaging activity in human peripheral lymphocytes, by using a sensitive method, fluorescence analysis of DNA unwinding (FADU). An aqueous turmeric component (Aq.T) was studied as a protective agent. TDS at one to 100 folds dilution induced 55% DNA damage at 20 min, while 12-O-tetradecanoylphorbol-13-acetate (TPA) at 10 ng/ml induced only 25% damage. Aq.T at 300 ng/microliter afforded 90% protection to DNA against TDS and 65% against TPA. The mechanism of Aq.T protection was investigated by using (i) inhibitors of arachidonate cascade, viz., indomethacin (28 microM), NDGA (10 microM), DBAP (36 microM), (ii) antioxidant enzymes viz., CAT (0.2 U/microliter), SOD (0.6 U/microliter), (iii) antioxidants--BHA, curcumin (40 microM), mixed gangliosides (20 nM) and protease inhibitor TLCK (100 microM). These compounds offered the following extents of protection to DNA against TDS: indomethacin--40%, NDGA--83%, DBAP--70%, SOD--38%, CAT--40%, BHA--38%, curcumin--60%, mixed gangliosides--88%, TLCK--85%. Against TPA as clastogenic agent, the extents of protection were: indomethacin--73%, NDGA--32%, DBAP--72%, SOD--60%, CAT, BHA-negligible, curcumin--23%, mixed gangliosides--60%, TLCK--59%. These results indicate that (i) TDS and TPA induce DNA damage possibly by different mechanisms, (ii) Aq.T is a more effective protectant against TDS whereas it is on par with other inhibitors against TPA.

Study Type : In Vitro Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2020 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.