Abstract Title:

[Resveratrol inhibits EGF-induced invasion of human lung adenocarcinoma A549 cells].

Abstract Source:

Zhongguo Fei Ai Za Zhi. 2010 Apr;13(4):287-91. PMID: 20677551

Abstract Author(s):

Ningyu Huang, Hong Lu, Lijun Chang, Hongwei Zhang, Hao Zhang, Guanwu Li

Article Affiliation:

Open Laboratory for Tumor Molecular Biology, Shantou University Medical College, Shantou 515041, China.

Abstract:

BACKGROUND AND OBJECTIVE: Invasion and metastasis are the primary causes of death in patients with pulmonary carcinoma. The epidermal growth factor (EGF) stimulates A549 cells invasion greatly through activating ERK and PI3K-Akt signaling pathway. The aim of this study is to elucidate the inhibitory effect of Resveratrol on EGF-induced invasive ability of A549 cells in vitro and explore the molecular mechanism.

METHODS: The cytotoxicity of Resveratrol was evaluated by methyl thiazolyltetrazolium (MTT) assay. Then, the A549 cells were treated with EGF and non-cytotoxic concentration of Resveratrol. The cells' invasion were detected by Boyden chamber assay; MMP-2 activity was determined by gelatine zymography assay; the changes of the related proteins were detected by Western blot.

RESULTS: Resveratrol was not toxic to A549 cells at the concentration between 0 to 30 microM. The invasion ability of EGF-induced A549 cells was decreased after treatment with 20 microM resveratrol for 24 h, accompanied by the inhibition of MMP-2 secretion. And the levels of p-ERK1/2, PI3K (within 6 h) were suppressed too.

CONCLUSION: 20 microM Resveratrol inhibits A549 cells' invasion possibly through the suppression of the activation of ERK and PI3K-Akt signaling pathways, subsequently exerting inhibitory effect on MMP-2.

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