Article Publish Status: FREE
Abstract Title:

Protective effect of fucoidan extract from Ecklonia cava on hydrogen peroxide-induced neurotoxicity.

Abstract Source:

J Microbiol Biotechnol. 2017 Nov 9. Epub 2017 Nov 9. PMID: 29121706

Abstract Author(s):

Seon Kyeong Park, Jin Yong Kang, Jong Min Kim, Sang Hyun Park, Bong Seok Kwon, Gun-Hee Kim, Ho Jin Heo

Article Affiliation:

Seon Kyeong Park


We evaluated the antioxidant activity and neuronal cell-protective effect of fucoidan extract from Ecklonia cava (FEC) on hydrogen peroxide (H2O2)-induced cytotoxicity in PC-12 and MC-IXC cells to assess its protective effect against oxidative stress. Antioxidant activities were examined using the ABTS radical scavenging activity and malondialdehyde (MDA)-inhibitory effect, and the results showed that FEC had significant antioxidant activity. Intracellular ROS content and neuronal cell viability were investigated using DCF-DA assay and MTT reduction assay. FEC also showed remarkable neuronal cell-protective effect compared to vitamin C as a positive control for both H₂O₂-treated PC-12 and MC-IXC cells. Based on the neuronal cell-protective effects, mitochondrial function was analyzed in PC-12 cells and FEC significantly restored mitochondrial damage by increasing the mitochondrial membrane potential (ΔΨm) and ATP levels and regulating the mitochondrial-mediated proteins (p-AMPK and BAX). Finally, the inhibitory effects against acetylcholinesterase (AChE), which is a critical hydrolyzing enzyme of the neurotransmitter acetylcholine (ACh) in the cholinergic system, were investigated (IC₅₀ value = 1.3 mg/mL) and showed a mixed (competitive/non-competitive) pattern of inhibition. Our findings suggest that EFC may be used as potential material for oxidative stress-induced neuronal damage by regulating the mitochondrial function and AChE inhibition.

Study Type : In Vitro Study

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