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Abstract Title:

UHPLC-QqQ-MS/MS identification, quantification of polyphenols from Passiflora subpeltata fruit pulp and determination of nutritional, antioxidant,α-amylase and α-glucosidase key enzymes inhibition properties.

Abstract Source:

Food Res Int. 2018 Jun ;108:611-620. Epub 2018 Apr 4. PMID: 29735097

Abstract Author(s):

Saravanan Shanmugam, Isla Alcântara Gomes, Marina Denadai, Bruno Dos Santos Lima, Adriano Antunes de Souza Araújo, Narendra Narain, Maria Terezinha Santos Leite Neta, Mairim Russo Serafini, Lucindo José Quintans-Júnior, Parimelazhagan Thangaraj

Article Affiliation:

Saravanan Shanmugam

Abstract:

The diabetic key enzymes inhibition, nutritional, antioxidant activity and bioactive compounds identification of Passiflora subpeltata fruit pulp were investigated. Fifteen polyphenolic compounds including protocatechuic acid, ferulic acid, vanillic acid, epicatechin, p-coumaric acid, cinnamic acid, eriodictyol and quercetin-3-glucoside were identified in the pulp of this species by using UHPLC-QqQ-MS/MS analysis. The total carbohydrates and crude protein contents in fruit pulp were 2.62 mg glucose equivalent/g sample fruit pulp and 8.80 mg BSA equivalent/g sample fruit pulp, respectively. The fresh fruit pulp of P. subpeltata contained high total phenolic (724.76 mg GAE/g sample) content and it revealed very high DPPH(ICof 5.667 μg/mL) and ABTS(6794.96 μM trolox equivalent/g sample) scavenging activities. In the key enzymes assays useful for diabetic inhibition the fresh fruit pulp characterized maximum inhibition of α-amylase and α-glucosidase ICof 18.69 and 32.63 μg/mL, respectively. Thus, these results lead to conclude that this fruit specie could be very useful source in nutraceutical products preparations for Type 2 diabetic suffering humans.

Study Type : In Vitro Study

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