n/a
Article Publish Status: FREE
Abstract Title:

Paeoniflorin blocks the proliferation of vascular smooth muscle cells induced by platelet‑derived growth factor‑BB through ROS mediated ERK1/2 and p38 signaling pathways.

Abstract Source:

Mol Med Rep. 2018 Jan ;17(1):1676-1682. Epub 2017 Nov 15. PMID: 29257209

Abstract Author(s):

Xianwei Fan, Jintao Wu, Haitao Yang, Lijie Yan, Shanling Wang

Article Affiliation:

Xianwei Fan

Abstract:

The proliferation of vascular smooth muscle cells (VSMCs) contributes to the development of vascular remodeling. In the present study, the effect of paeoniflorin (PAE) on the platelet derived growth factor‑BB (PDGF‑BB)‑induced proliferation of primary cultured rat VSMCs and its molecular mechanism was investigated. The toxicity was determined by the try pan blue exclusion test. Cell proliferation was determined using a CCK‑8 assay, DNA synthesis was assessed by measuring the incorporation ofBrdU. Cell cycle progression was determined using PI staining and fluorescence‑activated cell sorting. The level of intracellular reactive oxygen species (ROS) generation was assessed using dichlorodihydro fluorescein diacetate. mRNA expression was determined by reverse transcription quantitativepolymerase chain reaction. Changes of p38, JNK, ERK1/2 signaling pathways were determined by western blot analysis. Cell migration was detected by scratch assay. PAE was demonstrated to significantly inhibit VSMC proliferation induced by PDGF‑BB in a dose‑and time‑dependent manner without cell cytotoxicity. Thus, PAE blocked progression through the G0/G1 to Sphase of the cell cycle. Furthermore, inhibition of the cell cycle was associated with the inhibition of them RNA expression of cyclin D1, cyclin E, cyclin dependent kinase (CDK) 4 and CDK2 as well as with increased cyclin dependentkinase inhibitor 1A mRNA expression in PDGF‑BB‑stimulated VSMCs. Further studies showed that the beneficial effect of PAE on blocking VSMCs proliferation was related to the suppression of the ROS‑mediated extra cellular signal‑regulated kinase (ERK)1/2 and p38 signaling pathways, although PAE had no significant effect on the c‑Jun N‑terminal kinase signalling pathway. These results demonstrated that PAE suppressed PDGF‑BB‑induced VSMC proliferation through the ROS‑mediated ERK1/2 and p38 signaling pathways, suggesting that it may be a feasible therapy for vascular remodelling diseases.

Study Type : In Vitro Study

Print Options


Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2020 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.