n/a
Abstract Title:

[Paeoniflorin Improves Acute Lung Injury in Sepsis by Activating Nrf2/Keap1 Signaling Pathway].

Abstract Source:

Sichuan Da Xue Xue Bao Yi Xue Ban. 2020 Sep ;51(5):664-669. PMID: 32975081

Abstract Author(s):

Lin Ling, Jing Tong, Liang Zeng

Article Affiliation:

Lin Ling

Abstract:

Objective: To investigate the effect of paeoniflorin (PF) on sepsis-induced acute lung injury and its relationship with nuclear factor erythyroid 2-related factor 2 (Nrf2)/Kelch-like ECH2 associated protein 1 (Keap1) signaling pathway.

Methods: Cecal ligation and puncture (CLP) was used to induce the sepsis rat model. Rats were randomly divided into 5 groups (=10): sham group (Sham), model group (Model), low dose PF group (L-PF group, 50 mg/kg PF), high dose PF group (H-PF group, 150 mg/kg PF) and high dose PF+Nrf2 inhibitor group (H-PF+ML385 group, 150 mg/kg PF+30 mg/kg ML385). The severity of sepsis in rats was scored according to the improved severity scale of sepsis; the pathological changes of lung tissue were observed by HE staining; the activity of superoxide dismutase (SOD) in lung tissue was determined by xanthine oxidase method; the content of malondialdehyde (MDA) in lung tissue was measured by thiobarbituric acid method; and the levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in lung tissues were detected by ELISA. Western blot was used to detect the expression of Nrf2, Keap1 and HO-1 proteins in lung tissues.

Results: Compared with Sham group, the sepsis symptoms in Model group were more serious, severe inflammatory infiltration in lung tissue, macrophages and interstitial enlargement, and the contents of pro-inflammatory factors IL-1β, TNF-α, IL-6 and oxidative index MDA in lung tissues were significantly increased (<0.05), while the activity of antioxidant index SOD were significantly decreased (<0.05). The protein and mRNA expression levels of Nrf2 and HO-1 were significantly decreased (<0.05), while the protein and mRNA expression levels of Keap1 were markedly increased (<0.05). Compared with the Model group, the severity of sepsis in PF groups was decreased, the lung tissue injury was improved, the contents of IL-1β, TNF-α, IL-6 and MDA in lung tissue were significantly decreased (<0.05), the activity of SOD was markedly increased (<0.05), the protein and mRNA expression levels of Nrf2 and HO-1 were significantly increased (<0.05), and the expression levels of Keap1 protein and mRNA were significantly decreased (<0.05). Compared with the H-PF group, Nrf2 inhibitor ML385 significantly inhibited the improvement of PF on lung injury in sepsis rats.

Conclusion: PF can reduce oxidative stress and inflammation by activating Nrf2/Keap1 signaling pathway, and improve sepsis-induced acute lung injury.

Print Options


Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2021 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.