n/a
Article Publish Status: FREE
Abstract Title:

Molecular mechanism of the anti-diabetic activity of an identified oligosaccharide from.

Abstract Source:

Res Pharm Sci. 2020 Feb ;15(1):36-47. Epub 2020 Feb 20. PMID: 32180815

Abstract Author(s):

Gholamreza Bahrami, Seyed Shahram Miraghaee, Bahar Mohammadi, Mohammad Taher Bahrami, Gholamreza Taheripak, Samira Keshavarzi, Atefeh Babaei, Soraya Sajadimajd, Razieh Hatami

Article Affiliation:

Gholamreza Bahrami

Abstract:

Background and purpose: Because of the high prevalence, diabetes is considered a global health threat. Hence, the need for effective, cheap, and comfortable therapies are highly felt. In previous study, a novel oligosaccharide with strong anti-diabetic activity in the crude extract offruits, from thefamily, was identified. The present study was designed to ensure its efficacy usingandstudies.

Experimental approach: Crude extract and its purified oligosaccharide were prepared from corresponding herb. Adult male Wistar rats were randomly divided into four groups of 10 each, as follows: group 1, healthy control rats given only sterile normal saline; group 2, diabetic control rats received sterile normal saline; group 3, diabetic rats treated with crude extract of Rosa canina (40% w/v) by oral gavage for 8 weeks; group 4, diabetic rats treated with purified oligosaccharide of Rosa canina (2 mg/kg) by oral gavage for 8 weeks. After treatment, body weight, fasting blood glucose, serum insulin levels and islet beta-cell repair and proliferation were investigated. The possible cytoprotective action of oligosaccharide was evaluated. The effect of oligosaccharide on apoptosis and insulin secretion in cell culture media were examined. Real-time PCR was used to determine the expression level of some glucose metabolism-related regulator genes.

Findings / Results: In the animal model of diabetes, the insulin levels were increased significantly due to the regeneration of beta-cells in the islands of langerhans by the purified oligosaccharide.cell apoptosis examination showed that high concentration of oligosaccharide increased cell death, while at low concentration protected cells from streptozotocin-induced apoptosis. Molecular study showed that the expression ofandinsulin production genes were increased, leading to increased expression of insulin-dependent genes such asand. On the other hand, the expression of thegene, which is related to the glucose transporter 2, was significantly reduced due to insulin concentrations.

Conclusion and implications: The purified oligosaccharide fromwas a reliable anti-diabetic agent, which acted by increasing insulin production in beta-cells of the islands of Langerhans.

Study Type : Animal Study, In Vitro Study

Print Options


Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2020 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.