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Article Publish Status: FREE
Abstract Title:

MicroRNA 145 enhances chemosensitivity of glioblastoma stem cells to demethoxycurcumin.

Abstract Source:

Cancer Manag Res. 2019 ;11:6829-6840. Epub 2019 Jul 24. PMID: 31440081

Abstract Author(s):

Chunfa Qian, Bin Wang, Yuanjie Zou, Yansong Zhang, Xinhua Hu, Wenbo Sun, Hong Xiao, Hongyi Liu, Lei Shi

Article Affiliation:

Chunfa Qian

Abstract:

The presence of glioma stem cells (GSCs) is thought to be a key factor responsible for development of the incurable glioblastoma multiforme (GBM). GSCs are often displayed during chemotherapy resistance, except for demethoxycurcumin (DMC), a component of curcumin, which has been previously confirmed to inhibit GSCs proliferation and induce apoptosis.The objective of this study was to identify the main mechanism underlying anti-GSCs resistance by DMC.qRT-PCR was used to determine the expression of miR-145 in glioma patients and GSCs, and GSCs were transfected with miR-145 overexpressed vectors. Then, functional analyses (in vitro and in vivo) were performed to confirm the role of miR-145 and DMC in GSCs. Finally, related proteins were tested by immunohistochemistry and Western blot.miR-145 was atypically low-expressed miRNA in GSCs, and could enhance GSC chemosensitivity to DMC both in vitro and in vivo. Upregulation of miR-145 in GSCs resulted in increased cell growth inhibition and apoptosis to DMC. Further research on the mechanism demonstrated that the combined effects of miR-145 and DMC were involved in the miR-145/SOX2-Wnt/β-catenin pathway. Overexpression of SOX2 reduced GSC resistance to growth inhibition by miR-145+ DMC treatment. Our data strongly support an important role for miR-145 in enhancing GSC chemosensitivity to DMC by targeting the SOX2-Wnt/β-catenin axis.

Study Type : Animal Study, In Vitro Study

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Sayer Ji
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