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Abstract Title:

Maternal vitamin Bdeficiency in rats alters DNA methylation in metabolically important genes in their offspring.

Abstract Source:

Mol Cell Biochem. 2020 May ;468(1-2):83-96. Epub 2020 Mar 18. PMID: 32189172

Abstract Author(s):

Vinay Singh Tanwar, Sourav Ghosh, Satish Sati, Subhoshree Ghose, Lovejeet Kaur, Kalle Anand Kumar, K V Shamsudheen, Ashok Patowary, Meghna Singh, V Jyothi, Pujitha Kommineni, Sridhar Sivasubbu, Vinod Scaria, Manchala Raghunath, Rakesh Mishra, Giriraj Ratan Chandak, Shantanu Sengupta

Article Affiliation:

Vinay Singh Tanwar

Abstract:

Vitamin Bdeficiency is a critical problem worldwide and peri-conceptional deficiency of this vitamin is associated with the risk of complex cardio-metabolic diseases. Nutritional perturbations during these stages of development may lead to changes in the fetal epigenome. Using Wistar rat model system, we have earlier shown that low maternal Blevels are associated with low birth weight, adiposity, insulin resistance, and increased triglyceride levels in the offspring, which might predispose them to the risk of cardio-metabolic diseases in adulthood. In this study, we have investigated the effects of maternal Bdeficiency on genome-wide DNA methylation profile of the offspring and the effect of rehabilitation of mothers with Bat conception. We have performed methylated DNA immunoprecipitation sequencing of liver from pups in four groups of Wistar rats: Control (C), B-restricted (BR), B-rehabilitated at conception (BRC), and B-rehabilitated at parturition (BRP). We have analyzed differentially methylated signatures between the three groups as compared to controls. We have identified a total of 214 hypermethylated and 142 hypomethylated regions in the 10 kb upstream region of transcription start site in pups of B-deficient mothers, which are enriched in genes involved in fatty acid metabolism and mitochondrial transport/metabolism. Brehabilitation at conception and parturition is responsible for reversal of methylation status of many of these regions to control levels suggesting a causal association with metabolic phenotypes. Thus, maternal Brestriction alters DNA methylation of genes involved in important metabolic processes and influences the offspring phenotype, which is reversed by Brehabilitation of mothers at conception.

Study Type : Animal Study

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