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Abstract Title:

Mangiferin alleviates endoplasmic reticulum stress in acute liver injury by regulating the miR-20a/miR-101a-Nrf2 axis.

Abstract Source:

J Biochem. 2020 May 15. Epub 2020 May 15. PMID: 32413114

Abstract Author(s):

Shaoxun Li, Shuanghong Jin, Weilai Chen, Jiake Yu, Peipei Fang, Guangyao Zhou, Jie Li, Lingxiang Jin, Yiping Chen, Ping Chen, Chenwei Pan

Article Affiliation:

Shaoxun Li

Abstract:

: This study aimed to investigate the mechanism of mangiferin on regulating ER stress in acute liver injury. The mouse model of acute liver injury was established by injection of LPS/D-GalN. The primary mouse hepatocytes were stimulated with LPS to induce the in vitro model. The effect of miR-142-5p/212-5p on the luciferase activity of c-Myc 3'-UTR was assessed by luciferase reporter assay. ts: Mangiferin improved the liver function, inhibited the oxidative stress and ER stress, and down-regulated the expressions of miR-20a and miR-101a in LPS/D-GalN-induced mice and LPS-induced hepatocytes. The knockdown of miR-20a and miR-101a cooperatively alleviated ER stress of LPS-induced hepatocytes. miR-20a and miR-101a both targeted Nrf2 and the over-expression of miR-20a or miR-101a decreased Nrf2 protein level, while their silences increased Nrf2 protein level. The silence of miR-20a and miR-101a promoted Nrf2 expression and inhibited the ER stress in LPS-induced hepatocytes, while the knockdown of Nrf2 reversed these effects. The over-expression of miR-20a and miR-101a eliminated the effects of mangiferin on Nrf2 protein level and ER stress in LPS-induced hepatocytes, and Nrf2 overexpression altered these trends.

CONCLUSION: Our findings suggest that mangiferin alleviates ER stress in acute liver injury by regulating the miR-20a/miR-101a-Nrf2 axis.

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Sayer Ji
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