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Abstract Title:

Gold nanoparticle uptake is enhanced by estradiol in MCF-7 breast cancer cells.

Abstract Source:

Int J Nanomedicine. 2019 ;14:2705-2718. Epub 2019 May 1. PMID: 31118607

Abstract Author(s):

Carlos Lara-Cruz, Javier E Jiménez-Salazar, Marcela Arteaga, Michelle Arredondo, Eva Ramón-Gallegos, Nikola Batina, Pablo Damián-Matsumura

Article Affiliation:

Carlos Lara-Cruz

Abstract:

In the present study, we investigated the effects of 17β-estradiol (E) on membrane roughness and gold nanoparticle (AuNP) uptake in MCF-7 breast cancer cells.Estrogen receptor (ER)-positive breast cancer cells (MCF-7) were exposed to bare 20 nm AuNPs in the presence and absence of 1×10 M Efor different time intervals for up to 24 hrs. The effects of AuNP incorporation and Eincubation on the MCF-7 cell surface roughness were measured using atomic force microscopy (AFM). Endocytic vesicle formation was studied using confocal laser scanning microscopy (CLSM). Finally, the results were confirmed by hyperspectral optical microscopy.High-resolution AFM images of the surfaces of MCF-7 membranes (up to 250 nm) were obtained. The incubation of cells for 12 hrs with AuNP and Eincreased the cell membrane roughness by 95% and 30% compared with the groups treated with vehicle (ethanol) or AuNPs only, respectively. This effect was blocked by an ER antagonist (7α,17β-[9-[(4,4,5,5,5-Pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-triene-3,17-diol [ICI] 182,780). Higher amounts of AuNPs were localized inside MCF-7 cells around the nucleus, even after 6 hrs of Eincubation, compared with vehicle-treated cells. Endolysosome formation was induced by E, which may be associated with an increase in AuNP-uptake.Eenhances AuNP incorporation in MCF-7 cells by modulating of plasma membrane roughness and inducing lysosomal endocytosis. These findings provide new insights into combined nanotherapies and hormone therapies for breast cancer.

Study Type : In Vitro Study

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