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Abstract Title:

Aβ-glucan from Grifola frondosa effectively delivers therapeutic oligonucleotide into cells via dectin-1 receptor and attenuates TNFα gene expression.

Abstract Source:

Int J Biol Macromol. 2020 Apr 15 ;149:801-808. Epub 2020 Jan 23. PMID: 31982530

Abstract Author(s):

Hao Cui, Xinying Zhu, Zhengyi Huo, Bingbing Liao, Jingping Huang, Zhenxing Wang, Chunhui Song, Xiangguo Hu, Jianping Fang

Article Affiliation:

Hao Cui

Abstract:

Grifola frondosa is an edible and medicinal mushroom with great nutritional values and bioactivities. In the present study, a soluble homogeneousβ-glucan, GFPS, with high molecular mass of 5.42 × 10 Da was purified from the fruit bodies of Grifola frondosa using 5% cold NaOH. The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branchedat C-6 on every third residue. Our results indicated that GFPS had a triple helical structure and could form complex with polydeoxyadenylic acid (poly[A]). Further studies demonstrated that GFPS could interact with poly[A] moiety of a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60). This GFPS-based complex could incorporate TNFα-A60 into the macrophage cells via dectin-1 receptor and attenuate lipopolysaccharide-induced secretion of TNFα. Our results suggested that GFPS could be applied to deliver therapeutic oligonucleotides for the treatment of diseases such as inflammation and cancers.

Study Type : In Vitro Study

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