Article Publish Status: FREE
Abstract Title:

Antifungal activity, kinetics and molecular mechanism of action of garlic oil against Candida albicans.

Abstract Source:

Sci Rep. 2016 ;6:22805. Epub 2016 Mar 7. PMID: 26948845

Abstract Author(s):

Wen-Ru Li, Qing-Shan Shi, Huan-Qin Dai, Qing Liang, Xiao-Bao Xie, Xiao-Mo Huang, Guang-Ze Zhao, Li-Xin Zhang

Article Affiliation:

Wen-Ru Li


The antifungal activity, kinetics, and molecular mechanism of action of garlic oil against Candida albicans were investigated in this study using multiple methods. Using the poisoned food technique, we determined that the minimum inhibitory concentration of garlic oil was 0.35 μg/mL. Observation by transmission electron microscopy indicated that garlic oil could penetrate the cellular membrane of C. albicans as well as the membranes of organelles such as the mitochondria, resulting in organelle destruction and ultimately cell death. RNA sequencing analysis showed thatgarlic oil induced differential expression of critical genes including those involved in oxidation-reduction processes, pathogenesis, and cellular response to drugs and starvation. Moreover, the differentially expressed genes were mainly clustered in 19 KEGG pathways, representing vital cellular processes such as oxidative phosphorylation, the spliceosome, the cell cycle, and protein processing in the endoplasmic reticulum. In addition, four upregulated proteins selected after two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE) analysis were identified with high probability by mass spectrometry as putative cytoplasmic adenylate kinase, pyruvate decarboxylase, hexokinase, and heat shock proteins. This is suggestive of a C. albicans stress responses to garlic oil treatment. On the other hand, a large number of proteins were downregulated, leading to significant disruption of the normal metabolism and physical functions of C. albicans.

Study Type : In Vitro Study
Additional Links
Pharmacological Actions : Antifungal Agents : CK(403) : AC(237)
Additional Keywords : Plant Oils : CK(55) : AC(24)

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