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Abstract Title:

Changes in gene and protein expression in magnetic field-treated human glioma cells.

Abstract Source:

Toxicol Mech Methods. 2005 ;15(2):115-20. PMID: 20021071

Abstract Author(s):

R E Savage, M H Kanitz, W G Lotz, D Conover, E M Hennessey, W H Hanneman, F A Witzmann

Article Affiliation:

R E Savage

Abstract:

Because few cancer studies have examined protein profiles and genetic regulation from a single carcinogen exposure, the objective of this study was to determine genetic change via microarray and to evaluate whether that change was a precursor to cellular protein changes. In separate but experimentally identical studies, human glioma SF767 cells were exposed for 3 h to 60-Hz magnetic fields (sham or 1.2 muT). Microarray results suggested that magnetic field treatment resulted in the up-regulation of 5 genes, whereas 25 genes were down-regulated. The mean abundance of 10 identified proteins was altered following 1.2 muT exposure relative to sham (3 increase, 7 decrease). These studies suggest a limited but complicated response in the glioma cells to the magnetic field treatment.

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