n/a
Article Publish Status: FREE
Abstract Title:

Curcumin inhibited the growth and invasion of human monocytic leukaemia SHI-1 cellsby altering MAPK and MMP signalling.

Abstract Source:

Pharm Biol. 2020 Dec ;58(1):25-34. PMID: 31854220

Abstract Author(s):

Guohua Zhu, Qun Shen, Hong Jiang, Ou Ji, Lingling Zhu, Linyang Zhang

Article Affiliation:

Guohua Zhu

Abstract:

Curcumin, a polyphenolic compound extracted from the rhizome of the tropical plantL. (Zingiberaceae), has been considered as a cancer chemopreventive drug by American National Cancer Institute.To examine the effect of curcumin on acute monocytic leukaemia SHI-1 cells.The SHI-1 cells (1 × 10cells in 0.1 mL PBS) were injected subcutaneously into the right flanks of the female SCID mice. Curcumin dissolved in olive oil (15 and 30 mg/kg) was administered (i.p.) to mice once a day for 15 days while the control group received olive oil injection. Tumour proliferation and apoptosis were examined by PCNA, TUNEL and cleaved caspase-3 staining. The expression of MAPK, NF-κB, MMP9, MMP2 and vimentin were confirmed by RT-PCR, immunohistochemistry or western blotting.Administration of curcumin significantly inhibited tumour growth, as the tumour weight decreased from 0.67 g (control) to 0.47 g (15 mg/kg) and 0.35 g (30 mg/kg). Curcumin inhibited the expression of PCNA and increased the degree of TUNEL and cleaved caspase-3 staining in tumour tissue. The results of western blotting showed that curcumin treatment inhibited NF-κB and ERK signalling while activating p38 and JNK. Moreover, curcumin attenuated the mRNA transcription and protein expression of MMP2 and MMP9. Curcumin also suppressed the level of vimentin.Our study demonstrates that curcumin can inhibit the growth and invasion of human monocytic leukaemia, suggesting the possible use of curcumin for anti-metastasis in leukaemia and the value of determining its unique target.

Study Type : Animal Study

Print Options


Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2020 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.