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Article Publish Status: FREE
Abstract Title:

Exerts Anticancer Effect on Non-Small Cell Lung Cancer by Inhibiting Hedgehog Signaling via Suppression of TCTN3.

Abstract Source:

Integr Cancer Ther. 2020 Jan-Dec;19:1534735420923756. PMID: 32456485

Abstract Author(s):

Eunbi Jo, Hyun-Jin Jang, Lei Shen, Kyeong Eun Yang, Min Su Jang, Yang Hoon Huh, Hwa-Seung Yoo, Junsoo Park, Ik Soon Jang, Soo Jung Park

Article Affiliation:

Eunbi Jo

Abstract:

This study aimed to investigate the effect ofextract on the proliferation and apoptosis of non-small cell lung cancer (NSCLC) cells and determine the underlying mechanisms. We performed a CCK-8 assay to detect cell proliferation, detection of morphological changes through transmission electron microscopy (TEM), annexin V-FITC/PI double staining to analyze apoptosis, and immunoblotting to measure the protein expression of apoptosis and hedgehog signaling-related proteins, withtreated NSCLC cells. In this study, we first found thatreduced the viability and induced morphological disruption in NSCLC cells. The gene expression profiles indicated a reprogramming pattern of genes and transcription factors associated with the action of TCTN3 on NSCLC cells. We also confirmed that the-induced inhibition of TCTN3 expression affected the hedgehog signaling pathway. Immunoblotting indicated that-mediated TCTN3 downregulation induced apoptosis in NSCLC cells, involved in the serial activation of caspases. Moreover, we demonstrated that thenegatively modulated GLI1 transcriptional activity by suppressing SMO/PTCH1 signaling, which affects the intrinsic apoptotic pathway. When hedgehog binds to the PTCH1, SMO dissociates from PTCH1 inhibition at cilia. As a result, the active GLI1 translocates to the nucleus.clearly suppressed GLI1 nuclear translocation, leading to Bcl-2 and Bcl-xL down-regulation. These results suggested thatinduced NSCLC cell apoptosis, possibly through the downregulation of SMO/PTCH1 signaling and GLI1 activation via inhibition of TCTN3. Taken together, our findings provide new insights into the treatment of NSCLC using.

Study Type : In Vitro Study

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