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Article Publish Status: FREE
Abstract Title:

Capparis spinosa reduces Doxorubicin-induced cardio-toxicity in cardiomyoblast cells.

Abstract Source:

Avicenna J Phytomed. 2016 Sep-Oct;6(5):488-494. PMID: 27761417

Abstract Author(s):

Seyed Hadi Mousavi, Azar Hosseini, Elham Bakhtiari, Hassan Rakhshandeh

Article Affiliation:

Seyed Hadi Mousavi

Abstract:

OBJECTIVE: Doxorubicin (DOX) is an effective anticancer drug but its clinical application is limited because it induces apoptosis in cardiomyocytes and leads to permanent degenerative cardiomyopathy and heart failure possibly due to oxidative stress. Recent studies showed that Capparis spinosa (C. spinose) exhibits potent antioxidant activity. So, in this study, we explored the protective effect of hydro-alcoholic extract of C. spinosa against DOX-induced cytotoxicity in H9c2 cells.

MATERIALS AND METHODS: Cell viability was quantified by MTT assay. Apoptotic cells were determined using flow cytometry (sub-G1 peak) evaluation of DNA fragmentation following PI staining. Cells were cultured with 5μM DOX for 24 hr to induce cell damage. H9c2 cells were pretreated with different concentrations (6-200 μg/ml) of C. spinosa extract for 4 hr before DOX treatment in all trials.

RESULTS: Pretreatment with 25, 50, 100 and 200µg/ml of C. spinosa could increase the viability of H9C2 cells to 72.63±2.8% (p<0.05), 77.37±1.8% (p<0.05), 83.56±2.6% (p<0.001) and 90.9±0.5% (p<0.001) of control, respectively. Also, C. spinosa decreased apoptotic induction significantly, at the doses of 50µg/ml (p<0.05), 100µg/ml (p<0.01) and 200µg/ml (p<0.001).

CONCLUSION: Our results showed that C. spinosa could exert cardioprotective effects against DOX-induced toxicity that might be mediated via its antioxidant activity.

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