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Abstract Title:

Activation of bone marrow cells treated with Canova in vitro.

Abstract Source:

Cell Biol Int. 2006 Oct ;30(10):808-16. Epub 2006 Jul 7. PMID: 16884931

Abstract Author(s):

Ana Paula Ressetti Abud, Beatriz Cesar, Luiz Felipe Moscaleski Cavazzani, Carolina Camargo de Oliveira, Juarez Gabardo, Dorly de Freitas Buchi

Article Affiliation:

Ana Paula Ressetti Abud

Abstract:

Canova is a Brazilian complex homeopathic medication produced from Aconitum, Thuya, Bryonia, Lachesis and Arsenicum. Previous studies demonstrated that Canova induces up-regulation in numbers of leukocytes. The bone marrow microenvironment is composed of growth factors, stromal cells, extracellular matrix, and progenitor cells that differentiate into mature blood cells. As it is the major site of blood cell formation, we studied in vitro Canova effects on bone marrow cells of mice. Swiss mouse femurs were dissected, cleaned, and the marrow was flushed. The cells were plated, treated or not, incubated for different times and processed for light, scanning electron, and confocal microscopy, and also flow cytometry. The treatment did not modify the expression of the analyzed surface markers or cytokine production. All microscopy techniques showed that a monocytic lineage (CD11b(+)) and stromal cells (adherent cells) were activated by treatment. Canova also increased cell clusters over adherent cells, suggesting proliferation areas.

Study Type : Animal Study

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